三株弓形虫对昆明鼠致病性的比较研究

doi:10.11843/j.issn.0366-6964.2017.10.021

Abstract

Abstract:

Toxoplasma gondii is an obligate protozoan parasite with only one species, but it has rich genetic diversity with very different virulence. We chose three strains of the only species: Pru, BJ, VEG strains to compare their pathogenicity after intraperitoneal injection in Kunming mouse. We evaluated their virulence by clinical symptoms, serum antibody level, organ distribution of parasites and brain burden. Our results showed that mice symptoms infected with type Ⅱ was more significant than type Ⅲ infected group. Mice specific Toxoplasma antibody in serum was detected from the 4th day in type Ⅱ infection group, and remained increase after that until the 64th day, while type Ⅲ group got the peak earlier at the 32nd day. Interestingly, the three strains showed similar distribution features in mice organs: in early stage (the 1st day), parasites were detected in lung, kidney and muscle, then appeared in heart, lung, muscle and brain in mid-term (the 8th day), at last (the 32nd day) brain was the only detected organ. During infection, parasites were not be detected in spleen and liver. Our work successfully built up a Toxoplasma infection mice model of three strains and demonstrated their pathogenicity in detail. In addition, there was a certain similar regularity on mice organ distribution at different time among different Toxoplasma genotypes infection, which means organ tropism along time. Our results give reference for Toxoplasma pathogenicity and establishment of its animal infection model.

CLC Number:

S852.72

LI Yi, LI Xing, ZHOU Bing-jie, LI Mu-zi, MA Lei, LIU Qun, LIU Jing. Pathogenicity Comparison of Three Toxoplasma gondii Strains to Kunming Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1976-1982.

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References

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Pathogenicity Comparison of Three Toxoplasma gondii Strains to Kunming Mice

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